Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. Opportunity Identifier N/A. The BD Accuri™ C6 Plus personal flow cytometer is the newest generation of the BD Accuri™ platform. Our machine is still sitting in San Jose at BD over a year later because we haven't decided if it's worth paying for the repairs. A. Lin FSC-H, Channels 0 to 5 x 107 Lin SSC-H, Channels 0 to 2x 107 BD Accuri C6 Plus software features an intuitive user interface. Accuri™ C6 Plus BD Biosciences. Connect the cable to the socket at the back of the BD Accuri C6 and switch on the BD Accuri C6. Among CytoFlex and BD Accuri C6 Plus which one is best. To recreate BD Accuri C6 software plots in FlowJo with the same axes scaling as the original BD Accuri C6 software file, note the channel range used to view each parameter (Figure 1). In my Animal research laboratory, I want to procure a flow cytometer. Location Bethesda , MD 20814 USA. Examples of axes ranges used in a BD Accuri C6 software file. See manufactures web site for product specifications. BD Accuri® C6 flow cytometer, certain items must be considered to ensure proper data acquisition. • Collecting data in BD Accuri C6 Plus: Samples (and controls) can be collected in any position on the “Plate” in the software. 1. Accuri C6 Plus Condition: This BD Accuri C6 Flow Cytometer is in good working order. Andor J Kiss. The BD Accuri™ C6 Plus flow cytometer is easy to use, simple to maintain and affordable. Figure 1. Any settings will be remembered between samples until they are changed by the user. From basic to more challenging applications, the BD Accuri™ C6 Plus personal flow cytometer is a powerful benchtop tool that provides you with the versatility and the analytical power of a cytometer twice its size. Please complete the following information to request a demonstration of the BD Accuri™ C6 Plus flow cytometer. Name Lot Number Name Model 2nd Reporter Analyte Plex Components C4 Mouse IL … The item is untested and only what is pictured is included. 4 This filter transmits red laser light, which may damage the detector. Even new flow cytometry users find it so easy to learn and use that Figure 4. View online or download Bd Accuri C6 Instrument Manual BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express™ software and FlowJo™ software. Set Core Size .....78. Data acquisition and selection of viable single human sperm cells in the BD Accuri C6 Plus flow cytometer. Brasil Australia / New Zealand. The BD Accuri C6 is a powerful compact instrument for flow cytometery, and at 30lbs, it can be easily placed just about anywhere it’s needed, whether at a workbench or in a laminar hood. Figure S1. Fluid should accumulate in the tube. RELIABLE. Figure 1-1. Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. Designed from the ground up to be compact and easy to use, the BD Accuri C6 flow cytometer fits in any lab and is readily accessible to all researchers—experts and novices alike. (27.9 x 37.5 x 41.9 cm) (H x W x D) and weighs just 30 pounds (13.6 kg). The BD Accuri ™ C6 Plus is an easy-to-use personal flow cytometer equipped with a blue and a red laser and 4 fluorescence detectors. The BD Accuri C6 Plus Flow Cytometer brings users accessible, simple to maintain, affordable personal flow cytometry where they need it. BD Accuri C6 Plus FSC-A 1 PE-A FL4-A, FL3-A Instrument Debris Filter is active! NOTE: The BD Accuri CFlow Importer reads the FCS data directly from the BD Accuri CFlow file, so you do not have to export individual files. Select the BD Accuri CFlow file (.c6) that contains the data you want to view with FCS Express. Unless otherwise noted, 25 μL of each sample was collected from the same tube three times using the Medium fluidics setting (flow rate = 35 μL/ min, core size = 16 μm). Article Title: Interaction of allogeneic adipose tissue-derived stromal cells and unstimulated immune cells in vitro: the impact of cell-to-cell contact and hypoxia in the local milieu Article Snippet: ..Flow cytometric analysis was performed using an Accuri C6 with BD Accuri C6 Software (BD Biosciences). BD Accuri C6 Software Menus .....75 Figure B-1. BD Accuri™ C6 software was used for acquisition, analysis, and calculation of cell counts. 3. It is small enough to easily fit on a benchtop, and can be placed in a laminar flow hood. I have a BD Accuri C6 in my lab for four years, and met different kinds of problems, such as the valve problem a lots of people have been talking. Cite. 2. 3 See the BD Life Sciences technical bulletin Optical filter guidelines for fluorescent protein analysis with the BD Accuri™ C6 flow cytometer (applies to both the BD Accuri™ C6 and C6 Plus). BD Accuri C6 is ±20% of the expected value. The fixed, pre-optimized detector settings and the automated instrument QC allow for a simplified workflow. The BD Accuri™ is a personal bench-top flow cytometer designed to make flow-cytometry easier and more accessible. But are you aware that this small machine has been doing very big things? Bd Accuri C6 Pdf User Manuals. It is a personal flow cytometer that brings cell analysis within reach by easy to use, simple to maintain, and affordable. BD Accuri C6 with plate handler acquisition April 2016 Multi-well Auto Collect sample Important – only run this protocol after you have performed “Cytometer Setup/Verification” protocol on your BD Accuri C6 cytometer using particles. New generation Accuri makes flow cytometry even more within reach. Accuri™ Kits and Templates. (In this example, the file is FCS Express Import Demo.c6.) Analysis of immuno-oncology biomarkers using personal flow cytometry; Assessing NK cell cytotoxicity against tumor cells using personal flow cytometry; Automating dose-response cell cycle analysis with the BD CSampler™ Plus SIMPLE. BD Accuri C6 / plus -- Problem again and again? BD Accuri™ C6 Plus Filter Guide Product Information Sheets. (a) Representative density plot comparing side (SSC-A) versus forward scatter area (FSC-A). Start Date 18 Dec, 2020 (Yesterday) Due Date 02 Jan, 2021 (in 13 days) Opportunity Type Bid Notification. You are viewing a BD Accuri™ C6 Flow Cytometer by BD Biosciences. Gating to select sperm events and discriminate debris is indicated by the solid black line.b 8. The BD Accuri™ C6 has the sensitivity, reliability, and capabilities needed to bring flow cytometry to the next level. From: Federal Government (Federal) View Official Site. This small but powerful Accuri C6 comes computer loaded with BD Accuri™ C6 Plus software. For more information about the BD Accuri C6 Plus, see the Appendix at the end of this white paper. BD Accuri Cytometer. The BD Accuri C6 has a 1-minute UNCLOG cycle which may be run any time an obstruction is suspected. Biocompare is the leading resource for up-to-date product information, … The BD Accuri™ C6 flow cytometer offers an entirely new vision for the role of flow cytometry in life science research. The BD Accuri C6 Plus personal flow cytometer. The BD CSampler Plus automatic sampling accessory Small and easily transportable, the BD Accuri C6 Plus cytometer measures 11 x 14.75 x 16.5 in. The BD CSampler will auto- matically align itself and then remain in the home position (Figure 8 - 9). The BD Accuri™ C6 is a personal flow cytometer that brings flow cytometry within reach by being easy to use, simple to maintain, and affordable.The BD Accuri™ C6 flow cytometer along with the BD™ Cytometric Bead Array reagent sets, provides researchers … Canada United States South America. Save Share. o Set Run Limits- set the number of events to collect (in total or in a specific gate), or set the time Please see the exact video and photos below. To find it online, please go to bdbiosciences.com. Place an empty tube on the sample port and select the UNCLOG button in the workspace. BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. Custom Radio Button.....78 Figure B-2. Technical Bulletin Threshold and Analysis of Small Particles on the BD Accuri® C6 … Japan North America. VERSATILE. Ask BD Email Updates Contact Info Website Feedback. A Google Scholar search reveals more than 15000 citations associated with BD Accuri™ and the numbers are still growing. A8A4653E4DD76DCD Created by FCAP Array Infinite 2/59 Printed byAdministrator, 5/31/2017 5:58:32 PM. We sent the Accuri C6 Plus (received new Nov 2016 and this issue happened July 2018) into BD to be properly diagnosed and find out the cost for repair. BD Accuri C6 Plus is a compact, affordable, easy-to-use, transportable tool that can increase discovery right in the lab or in the field. This note discusses some of the basic concepts needed to perform small particle acquisition and analysis properly. By providing this information you also agree to receive BD Accuri News, our monthly electronic newsletter. 1 Recommendation. 29th Jan, 2018. The cycle may be repeated until fluid is .. Customer / Agency DEPT OF DEFENSE. The BD Accuri™ C6 Plus personal flow cytometer is the newest generation of the BD Accuri™ platform. Catalog Number BD Accuri C6 Plus This product is no longer available on Biocompare. If the winning bid plus applicable taxes, if any indicated, plus the buyer's premium equals to $5,000.00 or more, Wire Transfer must be used. Place the mat in position (Figure 10). This protocol also assumes a completed assay in a 96-well plate, with particles suspended in Run buffer. Science research be run any time an obstruction is suspected newest generation the! You also agree to receive BD Accuri News, our monthly electronic newsletter easy. Type Bid Notification this Filter transmits red laser and 4 fluorescence detectors any settings will be remembered samples! This white paper small enough to easily fit on a benchtop, and be. Is FCS Express Import Demo.c6. the UNCLOG button in the BD Accuri C6 software Menus..... 75 B-1. 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